Long-read sequencing vs short-read sequencing
Jul 26, 2021 · Long-read sequencing. Long-read sequencing technologies are capable of reading longer lengths, between 5,000 and 30,000 base pairs. Therefore, they immediately address one of the main challenges faced by short-read sequencing. They sequence a single molecule, eliminating amplification bias, and generate a reasonable length to overlap a sequence ...
What is multiplex PCR? - Front Line Genomics
Jul 26, 2021 · Multiplex PCR is a technique whereby PCR is used to amplify several different DNA sequences simultaneously. It is a type of target enrichment approach. It was first described in 1988 as a method to detect deletion mutations in the dystrophin gene – the largest known human gene. The dystrophin complex connects the cytoskeleton of muscle cells ...
Sample Preparation for NGS – A Comprehensive Guide
Aug 21, 2024 · Step #3: Amplification. This is an optional step, but it is usually required. It is dependent on the application of NGS and the sample size. Amplification becomes essential to obtain enough coverage for reliable sequencing for samples with small amounts of …
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How-to: perform a genome-wide association study (GWAS)
Sep 10, 2024 · Genome-wide association studies are commonly used in the genomics world to identify variants associated with a trait or disease.During a GWAS, researchers will identify areas of the genome that show a strong signal linked to the trait in question; that is, in the discovery cohort, areas or specific markers in which variation is more common than in the controls.
A brief history of Next Generation Sequencing (NGS)
Jul 26, 2021 · 2000: Lynx Therapeutics Company launched the first of the NGS technologies, called Massively parallel signature sequencing (MPSS). The company was later bought by Illumina. 2004: 454 Life Sciences marketed a new generation pyrosequencing technology, called the Roche GS20. It was the first NGS platform on the market.
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DNA Sequencing: How to Choose the Right Technology
Aug 6, 2021 · The global market for DNA sequencing is predicted to grow from $15.7 billion in 2021 to $37.7 billion by 2026. The rising prevalence of viral diseases, such as COVID-19, and the increasing cases of cancer globally are likely to drive genomic research and propel the industry at an even more rapid rate.
A History of Sequencing - Front Line Genomics
Apr 19, 2022 · Nucleic acid sequencing has become an integral part of modern biomedical research. The advances in sequencing technology, from its invention to modern-day equivalents, have been extraordinary. 2022 marks the 50 th anniversary of the sequencing of the first complete gene. It’s impressive to see how far science has come – so let’s take a look.
[Report] Advances in Single-Cell and Spatial Analysis 2022
Advances in Single-Cell & Spatial Analysis – 3rd Edition. Single-cell sequencing and analysis reveals a whole world of information contained in each individual cell. Spatial technologies allow that cellular information to be mapped back to its original tissue context. The past 12 months have seen a surge in the integration of these two ...